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DNA-protein interactions. Principles and protocols

DNA-protein interactions. Principles and protocols, Moss
Средняя: 4 (3 оценок)
Автор: 
Город: 
Издательство: 
Год издания: 
2001
Страниц: 
638стр.
Формат: 
pdf
Размер: 
3.54Mb

DNA-protein interactions. Principles and protocols:

  • Filter-Binding Assays
  • Electrophoretic Mobility Shift Assays for the Analysis of DNA-Protein Interactions
  • DNase I Footprinting
  • Footprinting with Exonuclease III
  • Hydroxyl Radical Footprinting
  • The Use of Diethyl Pyrocarbonate and Potassium
  • Permanganate as Probes for Strand Separation and Structural Distortions in DNA
  • Footprinting DNA-Protein Interactions in Native Polyacrylamide Gels by Chemical Nucleolytic Activity of 1,10-Phenanthroline-Copper
  • Uranyl Photofootprinting
  • Osmium Tetroxide Modification and the Study of DNA-Protein Interactions
  • Determination of a Transcription-Factor-Binding Site by Nuclease Protection Footprinting onto Southwestern Blots
  • Diffusible Singlet Oxygen as a Probe of DNA Deformation
  • Ultraviolet-Laser Footprinting
  • In Vivo DNA Analysis
  • Identification of Protein-DNA Contacts with Dimethyl Sulfate: Methylation Protection and Methylation Interference
  • Ethylation Interference
  • Hydroxyl Radical Interference
  • Identification of Sequence-Specific DNA-Binding Proteins by Southwestern Blotting
  • A Competition Assay for DNA Binding Using the Fluorescent Probe ANS
  • Site-Directed Cleavage of DNA by Linker Histone Protein-Fe(ll) EDTA Conjugates
  • Nitration of Tyrosine Residues in Protein-Nucleic Acid Complexes
  • Chemical Modification of Lysine by Reductive Methylation: A Probe of Residues Involved in DNA Binding
  • Limited Proteolysis of Protein-Nucleic Acid Complexes
  • Ultraviolet Crosslinking of DNA-Protein Complexes via 8-Azidoadenine
  • Site-Specific Protein-DNA Photocrosslinking: Analysis of Bacterial Transcription Initiation Complexes
  • Site-Directed DNA Photoaffinity Labeling of RNA Polymerase III Transcription Complexes
  • Use of Site-Specific Protein-DNA Photocrosslinking to Analyze the Molecular Organization of the RNA Polymerase II Initiation Complex
  • UV Laser-Induced Protein-DNA Crosslinking
  • Plasmid Vectors for the Analysis of Protein-Induced DNA Bending
  • Engineering Nucleic Acid-Binding Proteins by Phage Display
  • Genetic Analysis of DNA-Protein Interactions Using a Reporter Gene Assay in Yeast
  • Assays for Transcription Factor Activity
  • Assay of Restriction Endonucleases Using Oligonucleotides
  • Analysis of DNA-Protein Interactions by Intrinsic Fluorescence
  • Circular Dichroism for the Analysis of Protein-DNA Interactions
  • Calorimetry of Protein-DNA Complexes and Their Components
  • Surface Plasmon Resonance Applied to DNA-Protein Complexes
  • Reconstitution of Protein-DNA Complexes for Crystallization
  • Two-Dimensional Crystallization of Soluble Protein Complexes
  • Atomic Force Microscopy of DNA and Protein-DNA Complexes Using Functionalized Mica Substrates
  • Electron Microscopy of Protein-Nucleic Acid Complexes: Uniform Spreading of Flexible Complexes, Staining with a Uniform Thin Layer of Uranyl Acetate, and Determining Helix Handedness
  • Scanning Transmission Electon Microscopy of DNA-Protein Complexes
  • Determination of Nuleic Acid Recognition Sequences by SELEX
  • High DNA-Protein Crosslinking Yield with Two-Wavelength Femtosecond Laser Irradiation

В книге DNA-protein interactions. Principles and protocols представлено описание ДНК-белковых взаимодействий, их принципы и анализ, изучение ДНК-белковых взаимодействий, определение фактора транскрипции, идентификация белков, этилированию помех, определение последовательности конкретных ДНК-связывающих белков, огранический протеолиз белков, нуклеиновых комплексов, плазмидные векторы для анализа белков ДНК-индуцированных, генетический анализ ДНК-белковых взаимодействий, анализы для транскрипции активности фактора, рестриктаз с помощью олигонуклеотидов, калориметрия белка ДНК-комплексов и их компонентов, восстановление белка ДНК-комплексов для кристаллизации, атомно-силовая микроскопия ДНК и белков ДНК-комплексов, электронная микроскопия белково-нуклеиновых комплексов.