Nanoscience — Nanobiotechnology and Nanobiology

Опубликовано: 16 декабря 2013 | Книги по биотехнологии

Nanoscience — Nanobiotechnology and Nanobiology

Nanoscience — Nanobiotechnology and Nanobiology состоит из трех частей. Первый раздел Nanoscience — Nanobiotechnology and Nanobiology это Биологические нано—объекты:

  • Структурное и функциональное регулирование ДНК, в том числе Метилирования ДНК
  • Белково-липидные наноструктуры, включая биологические мембраны
  • Супермолекулярный комплекс ДНК, перенос генов в Vivo
  • Функционализированные неорганические наночастицы для биомедицинских применений, культивирование в пробирке
  • Жизнь наномашины
  • Теории эволюции Дарвина

Вторая часть книги Nanoscience — Nanobiotechnology and Nanobiology— это Методы нанобиотехнологии:

  • Оптические методы (флуоресцентная микроскопия и спектроскопия)
  • Нанобиотехнология и Медицина
  • Микроманипуляции
  • Поверхностные методы (спектроскопия, рефлектометрия)
  • Масс-спектрометрия
  • Источники ионизации биомолекул
  • Анализаторы
  • Комбинированные отделения жидкой фазы и масс-спектрометрия
  • Хроматографические методы
  • Методы электрофореза
  • Нанобиотехнология и системность данных
  • Молекулярная динамика

И заключением Nanoscience — Nanobiotechnology and Nanobiology является применение нанобиотехнологии:

  • Метод ПЦР в реальном времени (полимеразная цепная реакция)
  • Биосенсоры
  • Методы иммобилизации
  • Ферментные электроды
  • Биочипы
  • ДНК-микрочипы
  • Вирусные векторы для переноса генов
  • Фармацевтическое применения наночастиц
  • Активируемые наночастицы для лечения рака, нанобиотики
  • In Vitro эксперименты
  • In Vivo эксперименты
  • Медицинское, социальное и экономическое значение нанобиотехнологии.

Part 1 — Biological Nano-Objects

1. Structural and Functional Regulation of DNA:Geometry, Topology and Methylation:

  • Geometry of the DNA Double Helix
  • Conformation of DNA
  • Supercoiled DNA
  • Methylation of DNA
  • Methylation of Cytosine

2. Protein-Lipid Assemblyand Biomimetic Nanostructures:

  • Biological Membranes
  • Lipid Membranes
  • The Main Classes of Lipid Membranes
  • Lipid Polymorphism
  • Lipid Shapes
  • Models and Methods for Characterising Membranes
  • Liposomes
  • Supported Membranes
  • Suspended Membranes
  • Bilayer Lipid Membranes
  • Protein-Lipid Assembly
  • Two-Dimensional Organisation of Proteins on Lipid Surfaces
  • Reconstitution of Membrane Proteins in Supported Lipid Bilayers
  • Applications of Biomimetic Membranes in Nanobiotechnology
  • Bio-Optoelectronic Micro- and Nanosensors

3. Supramolecular Complexes of DNA:

  • Condensation and Protection of DNA
  • Circulation in a Multicellular Organism
  • Cell Adhesion and Crossing of the Plasma Membrane
  • Intracellular Circulation and Entry into the Nucleus
  • Polymolecular DNA Assemblies
  • Modification of Polyplexes and Lipoplexes for in Vivo Gene Transfer
  • Monomolecular DNA Assemblies (Nanoplexes)
  • Chemical Synthesis
  • Synthesis and Characterisation of Nanoplexes
  • Nanoplex Modification for in Vivo Gene Transfer

4. Functionalised Inorganic Nanoparticles for Biomedical Applications:

  • Synthesis and Chemical Surface Modification of Inorganic Nanoparticles     
  • Biological Tagging in Vitro and in Animals
  • In Vivo Applications

5. Living Nanomachines:

  • Force and Motion by Directed Assembly of Actin Filaments
  • Molecular Motors: Myosins and Kinesins
  • ATP Synthase

6. Aptamer Selection by Darwinian Evolution:

  • Some Theoretical Aspects of Molecular Evolution
  • Structural Features of Nucleic Acids
  • General Selection Principle
  • Chemical Modifications

Part 2 — Methods of Nanobiotechnology

7. Optical Tools:

  • Fluorescence Microscopy
  • Confocal Microscopy
  • Labels
  • Exogenous Probes
  • In Vivo Detection Systems
  • Basic Principles of in Vivo Optical
  • In Vitro Detection Systems
  • Biochips and Microarrays
  • Dynamics of Molecular Interactions
  • Fluorescence Correlation Spectroscopy (FCS)

8. Nanoforce and Imaging:

  • Molecular and Cellular Imaging Using AFM
  • Atomic Force Microscopy
  • Developments in Nanobiotechnology and Medecine
  • Surface Force Apparatus and Micromanipulation
  • Micromanipulation
  • Atomic Force Microscopy in Contact and Tapping Modes
  • Optical Tweezers
  • Biological Applications of Optical Tweezers
  • Magnetic Tweezers

9. Surface Methods:

  • Plasmon Resonance
  • Ellipsometry
  • Theory of Light and Polarisation
  • Optical Spectroscopy Using Waveguides
  • Vibrational Spectroscopy
  • Infrared Spectroscopy
  • Raman Spectroscopy
  • Prospects for Vibrational Spectroscopy in the Study of Nano-Objects
  • Brewster Angle Microscopy
  • Quartz Crystal Microbalance with Dissipation Monitoring
  • Grazing Incidence neutron and X-Ray Reflectometry

10. Mass Spectrometry:

  • Ionisation Sources for Biomolecules
  • Applications in Biology and Biochemistry
  • Analysers
  • Combined Liquid Phase Separation and Mass Spectrometry
  • Chromatographic Techniques
  • Electrophoretic Techniques
  • Nanotechnology for the MS Interface

11. Electrical Characterisation and Dynamics of Transport:

  • Ion Channels and the Patch-Clamp Technique
  • Amperometry
  • Macromolecular Transport Through natural and Artificial nanopores
  • Electrophoretic Techniques

12. Micro fluidics: Concepts and Applications to the Life Sciences:

  • Physics of Microfluidic Flows

13. Data Processing:

  • Nanobiotechnology and Data Systems
  • Nanobiotechnology
  • Representing Data
  • Correcting for Sensor Defects and Improving the Data
  • Data Extraction
  • Data Analysis

14. Molecular Dynamics:

  • Basic Principles of Molecular Dynamics
  • Potential Energy Function
  • Rigorous Treatment of Electrostatic Interactions
  • Some Properties Accessible to Simulation
  • Molecular Dynamics and Parallelisation

Part 3 – Applications of Nanobiotechnology

15. Real-Time PCR:

  • Polymerase Chain Reaction
  • Implementing Quantitative Real-Time PCR
  • Applications of Real-Time PCR

16. Biosensors. From the Glucose Electrode to the Biochip:

  • Bioreceptors
  • Immobilisation Methods
  • Biosensors with Electrochemical Detection
  • Enzyme Electrodes
  • Mass Transducer Biosensors
  • Enzyme Thermistors
  • Fibre Optic Biosensors
  • Biochips

17. DNA Microarrays:

  • Analysing the Transcriptome

18. Protein Microarrays:

  • Fabricating a Protein Array on a Flat Support
  • Formats for Protein Microarrays
  • Applications of Protein Microarrays

19. Cell Biochips:

  • Biochips for Analysing and Processing Living Cells

20. Lab on a Chip

21. Polyelectrolyte Multilayers

22. Biointcgrating Materials

23. Viral Vectors for in Vivo Gene Transfer

24. Pharmaceutical Applications of Nanoparticle Carriers

25. Activatable Nanoparticles for Cancer Treatment. Nanobiotix:

  • In Vitro Experiments
  • In Vivo Experiments

26. The Medical, Social, and Economic Stakes of Nanobiotechnology.

 

Метки: , , , , ,