DNA-protein interactions. Principles and protocols:
- Filter-Binding Assays
- Electrophoretic Mobility Shift Assays for the Analysis of DNA-Protein Interactions
- DNase I Footprinting
- Footprinting with Exonuclease III
- Hydroxyl Radical Footprinting
- The Use of Diethyl Pyrocarbonate and Potassium
- Permanganate as Probes for Strand Separation and Structural Distortions in DNA
- Footprinting DNA-Protein Interactions in Native Polyacrylamide Gels by Chemical Nucleolytic Activity of 1,10-Phenanthroline-Copper
- Uranyl Photofootprinting
- Osmium Tetroxide Modification and the Study of DNA-Protein Interactions
- Determination of a Transcription-Factor-Binding Site by Nuclease Protection Footprinting onto Southwestern Blots
- Diffusible Singlet Oxygen as a Probe of DNA Deformation
- Ultraviolet-Laser Footprinting
- In Vivo DNA Analysis
- Identification of Protein-DNA Contacts with Dimethyl Sulfate: Methylation Protection and Methylation Interference
- Ethylation Interference
- Hydroxyl Radical Interference
- Identification of Sequence-Specific DNA-Binding Proteins by Southwestern Blotting
- A Competition Assay for DNA Binding Using the Fluorescent Probe ANS
- Site-Directed Cleavage of DNA by Linker Histone Protein-Fe(ll) EDTA Conjugates
- Nitration of Tyrosine Residues in Protein-Nucleic Acid Complexes
- Chemical Modification of Lysine by Reductive Methylation: A Probe of Residues Involved in DNA Binding
- Limited Proteolysis of Protein-Nucleic Acid Complexes
- Ultraviolet Crosslinking of DNA-Protein Complexes via 8-Azidoadenine
- Site-Specific Protein-DNA Photocrosslinking: Analysis of Bacterial Transcription Initiation Complexes
- Site-Directed DNA Photoaffinity Labeling of RNA Polymerase III Transcription Complexes
- Use of Site-Specific Protein-DNA Photocrosslinking to Analyze the Molecular Organization of the RNA Polymerase II Initiation Complex
- UV Laser-Induced Protein-DNA Crosslinking
- Plasmid Vectors for the Analysis of Protein-Induced DNA Bending
- Engineering Nucleic Acid-Binding Proteins by Phage Display
- Genetic Analysis of DNA-Protein Interactions Using a Reporter Gene Assay in Yeast
- Assays for Transcription Factor Activity
- Assay of Restriction Endonucleases Using Oligonucleotides
- Analysis of DNA-Protein Interactions by Intrinsic Fluorescence
- Circular Dichroism for the Analysis of Protein-DNA Interactions
- Calorimetry of Protein-DNA Complexes and Their Components
- Surface Plasmon Resonance Applied to DNA-Protein Complexes
- Reconstitution of Protein-DNA Complexes for Crystallization
- Two-Dimensional Crystallization of Soluble Protein Complexes
- Atomic Force Microscopy of DNA and Protein-DNA Complexes Using Functionalized Mica Substrates
- Electron Microscopy of Protein-Nucleic Acid Complexes: Uniform Spreading of Flexible Complexes, Staining with a Uniform Thin Layer of Uranyl Acetate, and Determining Helix Handedness
- Scanning Transmission Electon Microscopy of DNA-Protein Complexes
- Determination of Nuleic Acid Recognition Sequences by SELEX
- High DNA-Protein Crosslinking Yield with Two-Wavelength Femtosecond Laser Irradiation
В книге DNA-protein interactions. Principles and protocols представлено описание ДНК-белковых взаимодействий, их принципы и анализ, изучение ДНК-белковых взаимодействий, определение фактора транскрипции, идентификация белков, этилированию помех, определение последовательности конкретных ДНК-связывающих белков, огранический протеолиз белков, нуклеиновых комплексов, плазмидные векторы для анализа белков ДНК-индуцированных, генетический анализ ДНК-белковых взаимодействий, анализы для транскрипции активности фактора, рестриктаз с помощью олигонуклеотидов, калориметрия белка ДНК-комплексов и их компонентов, восстановление белка ДНК-комплексов для кристаллизации, атомно-силовая микроскопия ДНК и белков ДНК-комплексов, электронная микроскопия белково-нуклеиновых комплексов.